beta Catenin antibody (AA 661-781)

Catalog No. ABIN738621

This anti-beta Catenin antibody is a Rabbit Polyclonal antibody detecting beta Catenin in WB, FACS, IF (cc), ELISA, IF (p), IHC (p) and IHC (fro). Suitable for Human, Mouse, Rat, Zebrafish (Danio rerio) and Pig. This Primary Antibody has been cited in 8+ publications.

Quick Overview for beta Catenin antibody (AA 661-781) (ABIN738621)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-beta Catenin Antibody

Binding Specificity: AA 661-781

Cross-Reactivity: Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Predicted Reactivity: Cow,Sheep,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Beta catenin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-beta Catenin antibody (ABIN738621)

Zhu, Shen, Xu, Chen, Huang: "Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats." in: Biochemical and biophysical research communications, Vol. 493, Issue 1, pp. 474-480, (2017) (PubMed).

Yang, Liu, Miao, Yang, Fu, Dou, Cai, Zong, Tan, Chen, Wang: "Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier." in: Oncotarget, Vol. 7, Issue 39, pp. 63839-63855, (2016) (PubMed).

Zheng, Liu, Li, Tang, Zhang, Tang: "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." in: Journal of neurosurgery, pp. 1-9, (2016) (PubMed).

Qi, Li, Xu, Chang, Shu, Zhou: "Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss." in: PeerJ, Vol. 4, pp. e2624, (2016) (PubMed).

Zhang, Shi, Song, Sun, Yang, Li: "BAMBI Promotes C2C12 Myogenic Differentiation by Enhancing Wnt/?-Catenin Signaling." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 17734-45, (2015) (PubMed).

Lu, Duan, Zhang, Wu, Wang: "Expression of Wnt3a, Wnt10b, ?-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." in: Acta odontologica Scandinavica, pp. 1-7, (2015) (PubMed).

Hu, Meng, Yu, Hu, Mao: "Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth." in: Oncotarget, Vol. 6, Issue 14, pp. 12174-87, (2015) (PubMed).

Lian, Duan, Wu, Li, Chen, Wang, Cai, Weng: "Expression and Clinical Significance of Von Hippel-Lindau Downstream Genes: Jade-1 and β-Catenin Related to Renal Cell Carcinoma." in: Urology, (2012) (PubMed).

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: Beta catenin

Background:

Synonyms: CTNNB, MRD19, armadillo, Catenin beta-1, Beta-catenin, CTNNB1, OK/SW-cl.35, PRO2286

Background: Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML.

Gene ID: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism