NOS1 antibody (AA 350-720)

Catalog No. ABIN7824477

This anti-NOS1 antibody is a Mouse Monoclonal antibody detecting NOS1 in WB, IHC, ELISA and ICC. Suitable for Human.

Quick Overview for NOS1 antibody (AA 350-720) (ABIN7824477)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: L121-25

Product Details anti-NOS1 Antibody

Binding Specificity: AA 350-720

Purpose: Anti-nNOS/NOS1 Antibody

Specificity: No off-targets reported

Cross-Reactivity: Mouse, Rat

Characteristics: Our Anti-nNOS/NOS1 mouse monoclonal primary antibody is produced in-house from hybridoma clone L121/25. It detects human, mouse, and rat nNOS/NOS1, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA, AT.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 350-720 of human NOS1 (accession number P29475) produced recombinantly in E. Coli

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 24 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: nNOS/NOS1

Background:

Synonyms: NOS1 (Brain nitric oxide synthase) (Constitutive NOS) (Neuronal NOS) (Peptidyl-cysteine S-nitrosylase NOS1)

Description Logic: Our Anti-nNOS/NOS1 mouse monoclonal primary antibody is produced from hybridoma clone L121/25. It detects human, mouse, and rat nNOS/NOS1, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA, AT.

Gene Name Alternatives: NOS1

Molecular Weight: 155-165 kDa for full-length protein, smaller fragments possible due to proteolysis and/or variable sample preparation

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction