SPTBN1 antibody (C-Term)

Catalog No. ABIN7824560

The Mouse Monoclonal anti-SPTBN1 antibody has been validated for WB, ELISA, IHC, IP and ICC. It is suitable to detect SPTBN1 in samples from Human.

Quick Overview for SPTBN1 antibody (C-Term) (ABIN7824560)

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTBN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: N385-21

Product Details anti-SPTBN1 Antibody

Binding Specificity: AA 1555-2137, C-Term

Purpose: Anti-Beta1-Spectrin Antibody

Specificity: No off-targets reported

Cross-Reactivity: Mouse, Rat

Characteristics: Our Anti-Beta1-spectrin mouse monoclonal primary antibody is produced in-house from hybridoma clone N385/21. It detects human, mouse, and rat Beta1-spectrin, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Purity: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 1555-2137 (C-terminal repeats 13 to 17) of human Beta1-spectrin (accession number P11277) produced recombinantly in E. Coli

Isotype: IgG2b

Application Details

Application Notes:

Dilution Range: IP: 5 μL

Dilution Range: ICC: 1:200

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: For maximum recovery of product, centrifuge the vial prior to removing the cap.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C.

Expiry Date: 24 months

Target Details for SPTBN1

Target: SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))

Alternative Name: Beta1-spectrin

Background:

Synonyms: Spectrin beta chain, erythrocytic (Beta-I spectrin)

Description Logic: Our Anti-Beta1-spectrin mouse monoclonal primary antibody is produced from hybridoma clone N385/21. It detects human, mouse, and rat Beta1-spectrin, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, ELISA.

Gene Name Alternatives: SPTB SPTB1

Molecular Weight: 250 kDa

UniProt: P11277

Pathways: Regulation of Actin Filament Polymerization, Protein targeting to Nucleus