Adenosine A2a Receptor antibody (AA 144-410)

Catalog No. ABIN7825254

This Rabbit Polyclonal antibody specifically detects Adenosine A2a Receptor in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for Adenosine A2a Receptor antibody (AA 144-410) (ABIN7825254)

Target: Adenosine A2a Receptor (ADORA2A)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Adenosine A2a Receptor Antibody

Binding Specificity: AA 144-410

Purpose: Anti-ADORA2A Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human ADORA2A shares 76.8%,75.3% amino acid (aa) sequence identity with mouse,rat ADORA2A,respectively.

Characteristics: Anti-ADORA2A Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ADORA2A recombinant protein (Position: N144-G410). Human ADORA2A shares 76.8% and 75.3% amino acid (aa) sequence identity with mouse and rat ADORA2A, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: ADORA2A

Background:

Background: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding.

Gene Full Name: adenosine A2a receptor

Molecular Weight: 45 kDa

Gene ID: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints