ATG5 antibody (AA 118-155)

Catalog No. ABIN7825269

This Rabbit Polyclonal antibody specifically detects ATG5 in IHC. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for ATG5 antibody (AA 118-155) (ABIN7825269)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-ATG5 Antibody

Binding Specificity: AA 118-155

Purpose: Anti-Zebrafish ATG5 Antibody

Characteristics: Anti-Zebrafish ATG5 Antibody catalog # AZF1RDC0. Tested in IHC applications. This antibody reacts with Zebrafish.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish ATG5 recombinant protein (Position: S118-L155).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry, 2-5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Gene ID: 494180

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy