SUPT16H antibody (AA 1-926)

Catalog No. ABIN7825372

The Rabbit Polyclonal anti-SUPT16H antibody is suitable to detect SUPT16H in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IF, FACS and ICC.

Quick Overview for SUPT16H antibody (AA 1-926) (ABIN7825372)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SUPT16H Antibody

Binding Specificity: AA 1-926

Purpose: Anti-SUPT16H Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human SUPT16H shares 98.6% amino acid (aa) sequence identity with mouse SUPT16H.

Characteristics: Anti-SUPT16H Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SUPT16H recombinant protein (Position: M1-E926). Human SUPT16H shares 98.6% amino acid (aa) sequence identity with mouse SUPT16H.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background:

Background: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, this gene encodes the 140 kDa subunit.

Gene Full Name: SPT16 homolog, facilitates chromatin remodeling subunit

Molecular Weight: 140 kDa

Gene ID: 11198

UniProt: Q9Y5B9

Pathways: Chromatin Binding