IDH3B antibody (AA 33-363)

Catalog No. ABIN7825491

This Rabbit Polyclonal antibody specifically detects IDH3B in IHC. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for IDH3B antibody (AA 33-363) (ABIN7825491)

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH3B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-IDH3B Antibody

Binding Specificity: AA 33-363

Purpose: Anti-Zebrafish IDH3B Antibody

Characteristics: Anti-Zebrafish IDH3B Antibody R8QPC9. Tested in IHC applications. This antibody reacts with Zebrafish.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish IDH3B recombinant protein (Position: T33-D363)

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry, 2-5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IDH3B

Target: IDH3B (Isocitrate Dehydrogenase 3 (NAD+) beta (IDH3B))

Alternative Name: IDH3B

Background: Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial is an enzyme that in humans is encoded by the IDH3B gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt: A0A2R89