MCM4 antibody (AA 343-659)

Catalog No. ABIN7825548

This Rabbit Polyclonal antibody specifically detects MCM4 in WB. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for MCM4 antibody (AA 343-659) (ABIN7825548)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCM4 Antibody

Binding Specificity: AA 343-659

Purpose: Anti-Zebrafish MCM4 Antibody

Characteristics: Anti-Zebrafish MCM4 Antibody catalog #AZQ6NZV2. Tested in WB applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish MCM4 recombinant protein (Position: M343-I659)

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4

Background: MCM4(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 4), also called CDC21, S. POMBE, HOMOLOG OF, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM4 gene is mapped to 8q11.21. The 864-amino acid MCM4 protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial.MCM4 encodes a subunit of the MCM2-7 complex(also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA