MARCKS antibody (C-Term)

Catalog No. ABIN7825570

The Rabbit Polyclonal anti-MARCKS antibody is suitable to detect MARCKS in samples from Human, Mouse and Rat. It has been validated for WB, IHC and FACS.

Quick Overview for MARCKS antibody (C-Term) (ABIN7825570)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MARCKS Antibody

Binding Specificity: C-Term

Purpose: Anti-MARCKS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MARCKS Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MARCKS.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background:

Background: The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Gene Full Name: myristoylated alanine rich protein kinase C substrate

Molecular Weight: 80 kDa

Gene ID: 4082

UniProt: P29966