PABPN1 antibody (Middle Region)

Catalog No. ABIN7825700

This anti-PABPN1 antibody is a Rabbit Polyclonal antibody detecting PABPN1 in WB, IF, IHC, IP, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for PABPN1 antibody (Middle Region) (ABIN7825700)

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PABPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PABPN1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-PABPN1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PABPN1 Antibody catalog # A02445-2. Tested in WB, IHC, IF, ICC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PABPN1. Human PABPN1 shares 100% amino acid (aa) sequence identity with mouse PABPN1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunofluorescence, 5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PABPN1

Target: PABPN1 (Poly A Binding Protein Nuclear 1 (PABPN1))

Alternative Name: PABPN1

Background: This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.

Molecular Weight: 50 kDa

Gene ID: 8106

UniProt: Q86U42