ASH2L antibody (AA 95-584)

Catalog No. ABIN7825813

The Rabbit Polyclonal anti-ASH2L antibody has been validated for IHC and IF. It is suitable to detect ASH2L in samples from Zebrafish (Danio rerio).

Quick Overview for ASH2L antibody (AA 95-584) (ABIN7825813)

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASH2L antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ASH2L Antibody

Binding Specificity: AA 95-584

Purpose: Anti-Zebrafish ASH2L Antibody

Characteristics: Anti-Zebrafish ASH2L Antibody catalog # AZA0A8M2B2E1. Tested in IHC, IF applications. This antibody reacts with Zebrafish.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived zebrafish ASH2L recombinant protein (Position: N95-G584).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry, 2-5 μg/mL, Zebrafish
Immunofluorescence, 5 μg/mL, Zebrafish

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ASH2L

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Alternative Name: ASH2L

Background: Set1/Ash2 histone methyltransferase complex subunit ASH2 is an enzyme that in humans is encoded by the ASH2L gene. The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator. While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4. These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity. MLL1 and MLL2 complexes contain the additional protein subunit, menin. Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4. MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis.

UniProt: A0A8M2B2E1