Recombinant Thrombomodulin antibody (AA 69-194)
Quick Overview for Recombinant Thrombomodulin antibody (AA 69-194) (ABIN7826102)
Target
See all Thrombomodulin (THBD) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 69-194
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Purpose
- CD141 antibody (141M)
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Immunogen
- Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein (exact sequence is proprietary)
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Isotype
- IgG1, kappa
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Lung: A strong staining should be seen in endothelial cells, and at least a weak to moderate membranous CD141 staining should be seen in alveolar macrophages.
Product Description/Background: CD141 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: Kidney: Epithelial cells should not stain for CD141.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Expiry Date
- 24 months
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- Thrombomodulin (THBD)
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Alternative Name
- CD141
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Background
- Gene Alias: AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin ™
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UniProt
- P07204
Target
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