Recombinant Caspase 3 antibody (Center)

Catalog No. ABIN7826109

This anti-Caspase 3 antibody is a Rabbit Monoclonal antibody detecting Caspase 3 in IHC. Suitable for Human.

Quick Overview for Recombinant Caspase 3 antibody (Center) (ABIN7826109)

Target: Caspase 3 (CASP3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV307

Product Details anti-Caspase 3 Antibody

Binding Specificity: Center

Purpose: Caspase-3 antibody (HMV307)

Immunogen: Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Stomach: At least a moderate caspase-3 positivity should be seen in surface epithelial cells.

Product Description/Background: Caspase-3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Stomach: Deep gastric glands and muscular cells must be caspase-3 negative.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: Gene Alias: caspase 3,CPP32,CPP32B,SCA-1

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation