Recombinant beta Catenin antibody
Quick Overview for Recombinant beta Catenin antibody (ABIN7826110)
Target
See all beta Catenin (CATNB) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Beta-Catenin antibody (578R)
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Immunogen
- Recombinant human CTNNB1 fragment
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Caput epididymis: A strong membranous β-Catenin staining should be seen in all epithelial cell types. In a fraction of the cells, an additional distinct nuclear staining must be seen.
Product Description/Background: Beta-Catenin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Placenta (mature): β-Catenin staining should be absent in the cells of the syncytiotrophoblast while it is strong in cells of the cytotrophoblast.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Expiry Date
- 24 months
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- beta Catenin (CATNB) (Catenin, beta (CATNB))
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Alternative Name
- Beta-Catenin
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Background
- Gene Alias: Catenin beta-1, Beta-catenin, Cadherin associated protein, beta 1 88 kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1
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UniProt
- P35222
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Pathways
- Peptide Hormone Metabolism
Target
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