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Recombinant beta Catenin antibody

This Rabbit Monoclonal antibody specifically detects beta Catenin in IHC. It exhibits reactivity toward Human.
Catalog No. ABIN7826110

Quick Overview for Recombinant beta Catenin antibody (ABIN7826110)

Target

See all beta Catenin (CATNB) Antibodies
beta Catenin (CATNB) (Catenin, beta (CATNB))

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This beta Catenin antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

578R
  • Purpose

    Beta-Catenin antibody (578R)

    Immunogen

    Recombinant human CTNNB1 fragment

    Isotype

    IgG
  • Application Notes

    IHC 1:100-1:200

    Comment

    Positive Control: Caput epididymis: A strong membranous β-Catenin staining should be seen in all epithelial cell types. In a fraction of the cells, an additional distinct nuclear staining must be seen.

    Product Description/Background: Beta-Catenin antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Placenta (mature): β-Catenin staining should be absent in the cells of the syncytiotrophoblast while it is strong in cells of the cytotrophoblast.

    Protocol

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

    Expiry Date

    24 months
  • Target

    beta Catenin (CATNB) (Catenin, beta (CATNB))

    Alternative Name

    Beta-Catenin

    Background

    Gene Alias: Catenin beta-1, Beta-catenin, Cadherin associated protein, beta 1 88 kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

    UniProt

    P35222

    Pathways

    Peptide Hormone Metabolism
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