Recombinant MCM2 antibody

Catalog No. ABIN7826115

The Rabbit Monoclonal anti-MCM2 antibody (Clone 502R) (ABIN7826115) specifically detects MCM2 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant MCM2 antibody (ABIN7826115)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 502R

Product Details anti-MCM2 Antibody

Purpose: MCM2 antibody (502R)

Immunogen: Recombinant fragment of human MCM2 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong nuclear MCM2 immunostaining should be seen in virtually all crypt base cells.

Product Description/Background: MCM2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: MCM2 immunostaining should be less intense or absent in surface epithelial cells and absent in most stroma cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2

Background: Gene Alias: DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDC like 1, cdc19, CDCL1, Cell devision cycle like 1, Cyclin like 1, DNA replication licensing factor MCM2, KIAA0030, Minichromosome maintenance complex component 2, Minichromosome maintenance deficient 2 (mitotin), Minichromosome maintenance protein 2 homolog, Mitotin, Nuclear protein BM28

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA