Recombinant MCM5 antibody

Catalog No. ABIN7826129

The Rabbit Monoclonal anti-MCM5 antibody has been validated for IHC. It is suitable to detect MCM5 in samples from Human.

Quick Overview for Recombinant MCM5 antibody (ABIN7826129)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 505R

Product Details anti-MCM5 Antibody

Purpose: MCM5 antibody (505R)

Immunogen: Recombinant human MCM5 fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong nuclear MCM5 immunostaining should be seen in virtually all crypt base cells.

Product Description/Background: MCM5 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: MCM5 immunostaining should be less intense or absent in surface epithelial cells and absent in most stroma cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: MCM5

Background: Gene Alias: Minichromosome maintenance complex component 5, CDC46, MGORS8, P1-CDC46

UniProt: P339922

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA