Recombinant PARP1 antibody

Catalog No. ABIN7826138

The Rabbit Monoclonal anti-PARP1 antibody has been validated for IHC. It is suitable to detect PARP1 in samples from Human.

Quick Overview for Recombinant PARP1 antibody (ABIN7826138)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HMV334

Product Details anti-PARP1 Antibody

Purpose: PARP1 antibody (HMV334)

Immunogen: Recombinant human PARP1 fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Placenta: A strong PARP1 positivity should be seen in all cell types except syncytiotrophoblast.

Product Description/Background: PARP1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Placenta: PARP1 positivity should be absent in the syncytiotrophoblast.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1

Background: Gene Alias: poly(ADP-ribose) polymerase 1 , ADPRT , ADPRT 1 , ADPRT1 , ARTD1 , PARP , PARP-1 , PPOL , pADPRT-1

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location