Recombinant ZAP70 antibody (AA 247-382)
Quick Overview for Recombinant ZAP70 antibody (AA 247-382) (ABIN7826139)
Target
See all ZAP70 AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Binding Specificity
- AA 247-382
-
Purpose
- ZAP-70 antibody (870R)
-
Immunogen
- Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein (exact sequence is proprietary)
-
Isotype
- IgG
-
-
-
-
Application Notes
- IHC 1:75-1:150
-
Comment
-
Positive Control: Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining.
Product Description/Background: ZAP-70 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres.
-
Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
-
Expiry Date
- 24 months
-
-
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
-
Alternative Name
- ZAP-70
-
Background
- Gene Alias: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa
-
UniProt
- P43403
-
Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
Target
-