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Recombinant ZAP70 antibody (AA 247-382)

This anti-ZAP70 antibody is a Rabbit Monoclonal antibody detecting ZAP70 in IHC. Suitable for Human.
Catalog No. ABIN7826139

Quick Overview for Recombinant ZAP70 antibody (AA 247-382) (ABIN7826139)

Target

See all ZAP70 Antibodies
ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This ZAP70 antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

870R
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    AA 247-382

    Purpose

    ZAP-70 antibody (870R)

    Immunogen

    Recombinant fragment (around aa 247-382) ofhuman ZAP70 protein (exact sequence is proprietary)

    Isotype

    IgG
  • Application Notes

    IHC 1:75-1:150

    Comment

    Positive Control: Tonsil: A large fraction of the lymphocytes in the interfollicular areas and few lymphocytes in the germinal centres should show a strong ZAP70 immunostaining.

    Product Description/Background: ZAP-70 antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Tonsil: ZAP70 immunostaining should be absent in epithelial tissues and the majority of cells in germinal centres.

    Protocol

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

    Expiry Date

    24 months
  • Target

    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

    Alternative Name

    ZAP-70

    Background

    Gene Alias: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70 kDa

    UniProt

    P43403

    Pathways

    TCR Signaling, Ubiquitin Proteasome Pathway
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