Recombinant KAP1 antibody (Center)
Quick Overview for Recombinant KAP1 antibody (Center) (ABIN7826150)
Target
See all KAP1 (TRIM28) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- Center
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Purpose
- KAP1 / TRIM28 antibody (HMV335)
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Immunogen
- Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.
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Isotype
- IgG
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anti-Tripartite Motif Containing 28 (TRIM28) (AA 576-835) antibody
TRIM28 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibodyTRIM28 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 4E6 unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (C-Term) antibodyVerified TRIM28 Reactivity: Human IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 Reactivity: Human WB, IF, IP, ICC, IHC (p), ChIP Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (N-Term) antibodyTRIM28 Reactivity: Human WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibodyTRIM28 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1D11 unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 22-291) antibodyTRIM28 Reactivity: Human WB, IHC, ELISA, IP Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) (AA 178-207), (N-Term) antibodyTRIM28 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB09760 unconjugated
anti-Tripartite Motif Containing 28 (TRIM28) antibodyTRIM28 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Colon: A strong nuclear TRIM28are staining should be seen in all cells.
Product Description/Background: KAP1 / TRIM28 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Testis: Spermatids are TRIM28 negative while all other cells show a strong nuclear TRIM28 staining of all cells.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Expiry Date
- 24 months
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- KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
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Alternative Name
- KAP1 / TRIM28
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Background
- Gene Alias: tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B
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UniProt
- Q13263
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Pathways
- Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
Target
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