Recombinant ASCL1 antibody

Catalog No. ABIN7827989

The Rabbit Monoclonal anti-ASCL1 antibody is suitable to detect ASCL1 in samples from Human. It has been validated for WB.

Quick Overview for Recombinant ASCL1 antibody (ABIN7827989)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A718

Product Details anti-ASCL1 Antibody

Purpose: Recombinant MASH1 Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: MASH1

Background: ASH,ASCL,HASH,MASH,bHLHa,ASCL1,ASH1,HASH1,MASH1,bHLHa46,Achaete scute complex homolog 1,Achaete scute complex homolog like 1,Achaete scute complex homologue 1,Achaete scute complex homologue like 1,Achaete scute complex like 1,Achaete scute family bHLH transcription factor 1,Achaete scute protein,Achaete-scute homolog 1,Ascl 1,Ash 1,ASH-1,Class A basic helix-loop-helix protein 46,Hash 1,Mammalian achaete scute homolog 1,Mammalian achaete scute homologue 1,Mash 1,MASH1, also known as achaete-scute homolog 1 (ASCL1), is a basic helix-loop-helix (BHLH) transcription factor which plays an essential role in the differentiation of neuroendocrine cells and neural tissues. MASH1 directly binds the E-box motif (5'-CANNTG-3') on promoters, with dimerization with other BHLH proteins required for efficient DNA binding. Acting as a pioneer transcription factor, MASH1 also accesses closed chromatin, allowing other factors to promote transcription of neuronal genes and activate neural pathways. Research studies have shown that knockdown of the MASH1 gene leads to inhibition of growth and induction of apoptosis in SCLC cells in vitro. Additionally, MASH1 is overexpressed in both classic SCLC as well as NSCLC with neuroendocrine features, suggesting its role in the pathogenesis of those malignancies. MASH1 plays a crucial role in promoting SCLC carcinogenesis by upregulating the expression of DLL3, a nonfunctioning Notch ligand, leading to inhibition of the Notch signaling pathway. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 25 kDa

Observed MW: 30 kDa The actual band is not consistent with the expectation.

UniProt: P50553

Pathways: Dopaminergic Neurogenesis