Recombinant AFT1 antibody

Catalog No. ABIN7827998

The Rabbit Monoclonal anti-AFT1 antibody is suitable to detect AFT1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for Recombinant AFT1 antibody (ABIN7827998)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: A562

Product Details anti-AFT1 Antibody

Purpose: Recombinant ATF1 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human ATF1 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000,IHC 1:100-1:1000,IF 1:50,FCM 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background: ATF,Atf1,ATF-1 is a bZIP transcription factor that is a member of the cyclic AMP (cAMP)-response element-binding protein (CREB) family. Like other members of the CREB family, ATF-1 mediates signals from numerous physiological stimuli and activates target genes through cAMP-response elements, resulting in the regulation of a broad array of cellular responses. ATF-1 can be phosphorylated at Ser63 by protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), and cyclin-dependent kinase 3 (CDK3), which recruits CBP/p300 and enhances transcriptional activity. ATF-1 expression levels have been found to be elevated in lymphomas and metastatic melanoma cells, where it contributes to tumor growth. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 29 kDa

Observed MW: 35 kDa The actual band is not consistent with the expectation.

UniProt: P81269

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades