Recombinant PRAS40 antibody

Catalog No. ABIN7828007

The Rabbit Monoclonal anti-PRAS40 antibody is suitable to detect PRAS40 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for Recombinant PRAS40 antibody (ABIN7828007)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: A589

Product Details anti-PRAS40 Antibody

Purpose: Recombinant PRAS40 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human PRAS40 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000,IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: PRAS40

Background: PRAS,AKT1 substrate,AKT1S,AKT1S1,Lobe,PRAS40,AKT1 substrate 1,PRAS40 is a proline-rich protein with a molecular weight of 40 kDa that acts as a substrate for the serine / threonine kinase Akt. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 27 kDa

Observed MW: 40 kDa The actual band is not consistent with the expectation.

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect