Recombinant BTK antibody (pTyr551)

Catalog No. ABIN7828051

This Rabbit Monoclonal antibody specifically detects BTK in FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant BTK antibody (pTyr551) (ABIN7828051)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BTK antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: A827

Product Details anti-BTK Antibody

Binding Specificity: pTyr551

Purpose: Recombinant Phospho-BTK (Tyr551) Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: FCM 1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: BTK

Background: IMD,AGMX,PSCTK,AGMX1,AT,ATK,BPK,IMD1,PSCTK1,XLA,BTK,Bruton tyrosine kinase,AGMX1,ATK,BPK,Bruton tyrosine kinase,IMD1,PSCTK1,XLA,Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains apleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation ofB cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 andTyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 withinthe SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btkat Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation. The PKC inhibitory signal islikely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling