Recombinant CPS1 antibody

Catalog No. ABIN7828062

The Rabbit Monoclonal anti-CPS1 antibody (Clone A622) (ABIN7828062) specifically detects CPS1 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Recombinant CPS1 antibody (ABIN7828062)

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CPS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: A622

Product Details anti-CPS1 Antibody

Purpose: Recombinant CPS1 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human CPS1 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000-1:5000,IHC 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for CPS1

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Alternative Name: CPS1

Background: CPS,carbamoyl-phosphate synthase,CPSASE,CPS1,CPSASE1,PHN,carbamoyl-phosphate synthase 1,carbamoyl-phosphate synthase 1,CPSASE1,PHN,Carbamoyl phosphate synthase,Carbamoyl phosphate synthase [ammonia],Carbamoyl phosphate synthase [ammonia] mitochondrial,Carbamoyl phosphate synthetase 1,Carbamoyl phosphate synthetase 1 mitochondrial,Carbamoyl phosphate synthetase I,Carbamoylphosphate synthase,Carbamoyl-phosphate synthase [ammonia],Carbamoylphosphate synthetase 1,Carbamoylphosphate synthetase I,Carbamoyl-phosphate synthetase I,CPS 1,CPSase 1,CPSase I,CPSM,mitochondrial,MS738,Carbamoyl phosphate synthetase 1 (CPS1), a rate-limiting enzyme in the urea cycle, catalyzes the conversion of ammonia and bicarbonate to carbamoyl phosphate in mitochondria. Non-small cell lung carcinoma (NSCLC) cells with oncogenicKRASand loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains pyrimidine/purine balance in these cancer cells. Silencing CPS1 reduces the pyrimidine to purine ratio and stalls DNA synthesis, leading to DNA damage and cancer cell death. In addition, the tumor suppressor p53 represses the expression of urea cycle enzymes CPS1, OTC, and ARG1 and, therefore, causes the accumulation of ammonia, suppressing cancer growth. Furthermore, research studies suggest that hypermethylation-mediated downregulation ofCPS1expression may contribute to the progression of normal hepatocytes to hepatocellular carcinoma (HCC). Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 165 kDa

Observed MW: 165 kDa

UniProt: P31327

Pathways: Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process