Recombinant CHD1 antibody

Catalog No. ABIN7828112

The Rabbit Monoclonal anti-CHD1 antibody is suitable to detect CHD1 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for Recombinant CHD1 antibody (ABIN7828112)

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CHD1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A646

Product Details anti-CHD1 Antibody

Purpose: Recombinant CHD1 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human CHD1 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for CHD1

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Alternative Name: CHD1

Background: CHD,chromodomain-helicase-DNA-binding protein,CHD1,chromodomain-helicase-DNA-binding protein 1,CHD1 belongs to the SNF2/RAD54 helicase family. CHD1 is an aTP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. CHD1 regulates polymerase II transcription. It is required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. CHD1 negatively regulates DNA replication. CHD1 is associated with histone deacetylase (HDAC) activity. CHD1 is required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. It functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. It is required for maintaining open chromatin and pluripotency in embryonic stem cells. In PTEN-deficient prostate and breast cancers, CHD1 depletion profoundly and specifically suppressed cell proliferation, cell survival and tumorigenic potential. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 197 kDa

Observed MW: 250 kDa The actual band is not consistent with the expectation.

UniProt: O14646

Pathways: Chromatin Binding