Recombinant CHD4 antibody

Catalog No. ABIN7828114

This Rabbit Monoclonal antibody specifically detects CHD4 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant CHD4 antibody (ABIN7828114)

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CHD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: A649

Product Details anti-CHD4 Antibody

Purpose: Recombinant CHD4 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human CHD4 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000-3000,IHC 1:50-100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for CHD4

Target: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Alternative Name: CHD4

Background: CHD,CHD4,CHD-4,Mi-2b,Mi2-BETA,SIHIWES,Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 218 kDa

Observed MW: 280 kDa The actual band is not consistent with the expectation.

UniProt: Q14839