Recombinant Cyclin B1 antibody

Catalog No. ABIN7828138

This Rabbit Monoclonal antibody specifically detects Cyclin B1 in WB, IF and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant Cyclin B1 antibody (ABIN7828138)

Target: Cyclin B1 (CCNB1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: A546

Product Details anti-Cyclin B1 Antibody

Purpose: Recombinant Cyclin B1 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human Cyclin B1 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000,IF 1:50,IP 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background: G2 mitotic specific cyclin B,G2/mitotic-specific cyclin-B,Cyclin B,CCNB,CCNB1,Cyclin B1,CCNB 1,G2 mitotic specific cyclin B1,G2/mitotic-specific cyclin-B1,Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 55 kDa

Observed MW: 55 kDa

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase