Recombinant EGFR antibody (pTyr1173)

Catalog No. ABIN7828180

This Rabbit Monoclonal antibody specifically detects EGFR in WB and IF. It exhibits reactivity toward Human.

Quick Overview for Recombinant EGFR antibody (pTyr1173) (ABIN7828180)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: A825

Product Details anti-EGFR Antibody

Binding Specificity: pTyr1173

Purpose: Recombinant Phospho-EGFR (Tyr1173) Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000,IF 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: PIG,HER,Proto-oncogene c-ErbB,Receptortyrosine-protein kinase erbB,Receptor tyrosine-protein kinase erbB,NISBD,EGFR,ERBB,ERBB1,HER1,NISBD2,PIG61,mENA,ErbB-1,Receptortyrosine-protein kinase erbB-1,Epidermal growth factor receptor,Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1, NISBD2,epidermal growth factor receptor,ERBB,ERBB1,HER1,mENA,NISBD2,PIG61,Avian erythroblastic leukemia viral (v erb b) oncogene homolog,Cell growth inhibiting protein 40,Cell proliferation inducing protein 61,EGF R,Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog),Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian),ErbB1,ERBB,erb-b2 receptor tyrosine kinase 1,Errp,Oncogen ERBB,Receptor tyrosine protein kinase ErbB 1,SA7,Species antigen 7,Urogastrone,v-erb-b Avian erythroblastic leukemia viral oncogen homolog,wa2,Wa5,The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand bindingresults in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation.Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the activestate enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domainof PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation. TheGRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide adocking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specificserine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylatedby CaM kinase II, mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 134 kDa

Observed MW: 170 kDa The actual band is not consistent with the expectation.

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins