Recombinant EIF3B antibody

Catalog No. ABIN7828188

The Rabbit Monoclonal anti-EIF3B antibody (Clone A212) (ABIN7828188) specifically detects EIF3B in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant EIF3B antibody (ABIN7828188)

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EIF3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: A212

Product Details anti-EIF3B Antibody

Purpose: Recombinant eIF3B Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human eIF3B1 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000,IHC 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for EIF3B

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Alternative Name: eIF3B

Background: PRT,EIF3S,EIF3B,EIF3-ETA,EIF3-P110,EIF3-P116,EIF3S9,PRT1,RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. UniRule annotation4 Publications (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 92 kDa

Observed MW: 116 kDa The actual band is not consistent with the expectation.

UniProt: P55884

Pathways: Ribonucleoprotein Complex Subunit Organization