Recombinant HSP27 antibody (pSer78)

Catalog No. ABIN7828250

The Rabbit Monoclonal anti-HSP27 antibody is suitable to detect HSP27 in samples from Human. It has been validated for WB, IHC, IF and IP.

Quick Overview for Recombinant HSP27 antibody (pSer78) (ABIN7828250)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: A811

Product Details anti-HSP27 Antibody

Binding Specificity: pSer78

Purpose: Recombinant Phospho-HSP27 (Ser78) Monoclonal Antibody

Purification: Protein A purified

Immunogen: phosphorylated human HSP27 (Ser78) peptide

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000,IHC 1:100-1:500,IF 1:50,IP 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Background: SRP,HSPB,HEL-S,HSP,HSPB1,CMT2F,HEL-S-102,HMN2B,HS.76067,HSP27,HSP28,Hsp25,SRP27,28 kDa heat shock protein,DKFZp586P1322,epididymis secretory protein Li 102,Estrogen regulated 24 kDa protein,Estrogen-regulated 24 kDa protein,Heat shock 25 kDa protein 1,Heat shock 27 kDa protein,Heat shock 27kD protein 1,Heat shock 27 kDa protein,Heat shock 27 kDa protein 1,Heat shock 28 kDa protein 1,Heat Shock Protein 27,Heat shock protein beta 1,Heat shock protein beta-1,heat shock protein family B (small) member 1,Hsp 25,HSP 27,Hsp 28,Hsp B1,Stress responsive protein 27,Stress-responsive protein 27,Hsp27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers. It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 23 kDa

Observed MW: 27 kDa The actual band is not consistent with the expectation.

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling