Recombinant MPHOSPH8 antibody

Catalog No. ABIN7828368

The Rabbit Monoclonal anti-MPHOSPH8 antibody (Clone A305) (ABIN7828368) specifically detects MPHOSPH8 in WB. The antibody is reactive with Mouse samples.

Quick Overview for Recombinant MPHOSPH8 antibody (ABIN7828368)

Target: MPHOSPH8 (M-Phase phosphoprotein 8 (MPHOSPH8))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MPHOSPH8 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A305

Product Details anti-MPHOSPH8 Antibody

Purpose: Recombinant MPP8 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human MPP8 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for MPHOSPH8

Target: MPHOSPH8 (M-Phase phosphoprotein 8 (MPHOSPH8))

Alternative Name: MPP8

Background: MPP,Mphosph,MPP8,MPHOSPH8,Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression. Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2. Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites, these can be situated within the coding region of the gene. Mediates down-regulation of CDH1 expression. Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 110 kDa

Observed MW: 125 kDa The actual band is not consistent with the expectation.

UniProt: Q99549