Recombinant PHF8 antibody

Catalog No. ABIN7828452

The Rabbit Monoclonal anti-PHF8 antibody (Clone A514) (ABIN7828452) specifically detects PHF8 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant PHF8 antibody (ABIN7828452)

Target: PHF8 (PHD Finger Protein 8 (PHF8))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PHF8 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A514

Product Details anti-PHF8 Antibody

Purpose: Recombinant PHF8 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human PHF8 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for PHF8

Target: PHF8 (PHD Finger Protein 8 (PHF8))

Alternative Name: PHF8

Background: ZNF,Phf,KIAA,KIAA1111,ZNF422,PHF8,PHF8 is a histone lysine demethylase that functions as a transcriptional activator by specifically demethylating a number of repressive histone methylation marks: mono- and Dimethyl-histone H3 Lys9 (H3K9me1 and H3K9me2), Dimethyl-histone H3 Lys27 (H3K27me2) and Monomethyl-histone H4 Lys20 (H4K20me1). PHF8 contains an N-terminal zinc finger-like PHD domain that binds Trimethylated histone H3 Lys4 (H3K4Me3) and a C-terminal jumonji domain that is responsible for the demethylase activity. PHF8 is highly expressed in prostate cancer, laryngeal squamous cell carcinoma, and human non-small-cell lung cancer (NSCLC). Its expression is predictive of poor survival. Overexpression of PHF8 increases cell proliferation and cell motility, while silencing of PHF8 reduces cell proliferation, migration, and invasion. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 118 kDa

Observed MW: 140 kDa The actual band is not consistent with the expectation.

UniProt: Q9UPP1