Recombinant RAG2 antibody

Catalog No. ABIN7828521

The Rabbit Monoclonal anti-RAG2 antibody (Clone A350) (ABIN7828521) specifically detects RAG2 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant RAG2 antibody (ABIN7828521)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAG2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A350

Product Details anti-RAG2 Antibody

Purpose: Recombinant RAG2 Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant human RAG2 fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000-1:10000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Alternative Name: RAG2

Background: RAG,RAG2,RAG-2,recombination activating 2,recombination activating gene 2,Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 62 kDa

Observed MW: 62 kDa

UniProt: P55895

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response