Recombinant RNF40 antibody

Catalog No. ABIN7828533

The Rabbit Monoclonal anti-RNF40 antibody (Clone A775) (ABIN7828533) specifically detects RNF40 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant RNF40 antibody (ABIN7828533)

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RNF40 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A775

Product Details anti-RNF40 Antibody

Purpose: Recombinant RNF40 Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for RNF40

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Alternative Name: RNF40

Background: RBP,RNF,KIAA,RNF40,BRE1B,RBP95,STARING,KIAA0661,In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1. Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin. Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified. These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Researchers have shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways. Indeed, investigators have shown that loss of RNF20-RNF40 function promotes replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 114 kDa

Observed MW: 140 kDa The actual band is not consistent with the expectation.

UniProt: O75150