Recombinant SMAD2 antibody (pSer255)

Catalog No. ABIN7828569

The Rabbit Monoclonal anti-SMAD2 antibody (Clone A818) (ABIN7828569) specifically detects SMAD2 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant SMAD2 antibody (pSer255) (ABIN7828569)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A818

Product Details anti-SMAD2 Antibody

Binding Specificity: pSer255

Purpose: Recombinant Phospho-Smad2 (S255) Monoclonal Antibody

Purification: Protein A purified

Immunogen: Recombinant Smad2 (phospho S255) fragment

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Smad2

Background: MADH,MADR,hMAD,SMAD,hSMAD,JV18,JV18-1,MADH2,MADR2,hMAD-2,hSMAD2,Smad2,Drosophila,homolog of,hSMAD family member 2,JV181,MAD,MAD homolog 2,MAD Related Protein 2,Mad-related protein 2,MGC22139,MGC34440,Mother against DPP homolog 2,Mothers against decapentaplegic,Mothers against decapentaplegic homolog 2,Mothers against DPP homolog 2,OTTHUMP00000163489,Sma and Mad related protein 2,Sma- and Mad-related protein 2 MAD,SMAD 2,SMAD family member 2,SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation, which increases transcriptional activity and stability, and is blocked by calmodulin. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 52 kDa

Observed MW: 58 kDa The actual band is not consistent with the expectation.

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus