Recombinant TSC1 antibody

Catalog No. ABIN7828644

The Rabbit Monoclonal anti-TSC1 antibody (Clone A773) (ABIN7828644) specifically detects TSC1 in WB. The antibody is reactive with Mouse and Rat samples.

Quick Overview for Recombinant TSC1 antibody (ABIN7828644)

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TSC1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: A773

Product Details anti-TSC1 Antibody

Purpose: Recombinant Hamartin Monoclonal Antibody

Purification: Protein A purified

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Alternative Name: Hamartin

Background: KIAA,TSC1,LAM,TSC,hamartin,KIAA0243,Tuberous sclerosis complex (TSC) is an autosomal dominant disorder that causes symptoms including hamartomas in brain, kidney, heart, lung and skin. The tumor suppressor genes TSC1 and TSC2 encode hamartin and tuberin, respectively. Hamartin and tuberin form a functional complex and are involved in numerous cellular activities such as vesicular trafficking, regulation of the G1 phase of the cell cycle, steroid hormone regulation, Rho activation and anchoring neuronal intermediate filaments to the actin cytoskeleton. The combination of genetic, biochemical and cell-biological studies demonstrate that the tuberin/hamartin complex functions as a GTPase-activating protein for the Ras-related small G protein Rheb and thus inhibits targets of rapamycin including mTOR. Cells lacking hamartin or tuberin fail to inhibit phosphorylation of S6 kinase resulting in the activation of S6 ribosomal protein's translation of 5'TOP mRNA transcripts. Hamartin is phosphorylated by CDK1 (cdc2) at Thr417, Ser584 and Thr1047 in cells in G2/M phase of the cell cycle. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 130 kDa

Observed MW: 160 kDa The actual band is not consistent with the expectation.

UniProt: Q92574

Pathways: RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation