Recombinant YAP1 antibody (pSer127)

Catalog No. ABIN7828681

This Rabbit Monoclonal antibody specifically detects YAP1 in WB, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant YAP1 antibody (pSer127) (ABIN7828681)

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This YAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: A804

Product Details anti-YAP1 Antibody

Binding Specificity: pSer127

Purpose: Recombinant Phospho-YAP1 (Ser127) Monoclonal Antibody

Purification: Protein A purified

Immunogen: phosphorylated human YAP1 (Ser1270) peptide

Isotype: IgG, kappa

Application Details

Application Notes: WB 1:500-1:2000,IHC 1:100-1:200,IF 1:50,FCM 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for YAP1

Target: YAP1 (Yes-Associated Protein 1 (YAP1))

Alternative Name: YAP1

Background: COB,COB1,YAP,YAP2,YAP65,YKI,YAP1,65 kDa Yes associated protein,65 kDa Yes-associated protein,Basic protease inhibitor,BPTI,Pancreatic Trypsin Inhibitor,Trasylol,YAp 1,YAP 65,Yes associated protein 1,Yes associated protein 1 65 kDa,Yes associated protein 2,yes associated protein beta,yes -associated protein delta,Yorkie homolog,Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molecular Weight:

Calculated MW: 54 kDa

Observed MW: 70-100 kDa The actual band is not consistent with the expectation.

UniProt: P46937

Pathways: MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha