BrdU antibody (Biotin)

Catalog No. ABIN7830275

The Mouse Monoclonal anti-BrdU antibody is suitable to detect BrdU. It has been validated for IHC (p), IHC (fro) and ICFC.

Quick Overview for BrdU antibody (Biotin) (ABIN7830275)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: Bu20a

Product Details anti-BrdU Antibody (Biotin)

Purpose: Anti-BrdU Biotin

Characteristics: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Bromodeoxyuridine conjugated to BSA

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical

Background: Optional[NCBI Full Gene Name]: 5-bromodeoxyuridine

Gene ID: 35