tdTomato antibody (DyLight 680)

Catalog No. ABIN7830305

The Goat Polyclonal anti-tdTomato antibody is suitable to detect tdTomato in samples from Discosoma and Tomato. It has been validated for IF, IHC (fro) and WB.

Quick Overview for tdTomato antibody (DyLight 680) (ABIN7830305)

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reactivity: Discosoma, Tomato

Host: Goat

Clonality: Polyclonal

Conjugate: This tdTomato antibody is conjugated to DyLight 680

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Product Details anti-tdTomato Antibody (DyLight 680)

Purpose: Anti-tdTomato, DyLight®680

Sequence: MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Characteristics: Red Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for tdTomato

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Alternative Name: tdTomato

Background:

Goat polyclonal antibody to tdTomato (red fluorescent protein) conjugated to DyLight® 680. tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.

Cherry fluorescent protein, DsRed, mCherry, red fluorescent protein, RFP antibody.