The Mouse Monoclonal anti-Blue Fluorescent Protein antibody (ABIN7833179) specifically detects Blue Fluorescent Protein in ELISA.
The antibody is reactive with Aequorea victoria samples.
Recommended antibody concentration: 0.5 mg/mL (when dissolved at 0.5 mg/mL, the BSA concentration will be 1 % ). Recommended solvent, 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05 %) is recommended for long term storage. •For a 0.5 mg/mL antibody concentration in 1 % BSA, dissolve in 200 μL buffer.
Buffer
Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Handling Advice
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Storage
4 °C
Storage Comment
Store lyophilized/reconstituted at 2-8°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Target
Blue Fluorescent Protein (BFP)
Alternative Name
Blue Fluorescent Protein
Background
Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.