ICE1 antibody

Catalog No. ABIN7833328

The Rabbit Polyclonal anti-ICE1 antibody is suitable to detect ICE1 in samples from Arabidopsis thaliana. It has been validated for WB.

Quick Overview for ICE1 antibody (ABIN7833328)

Target: ICE1

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-ICE1 | Inducer of CBF expression 1

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Solanum lycopersicum

Predicted Reactivity: Brassica napus,Brassica rapa,Capsella bursa-pastoris,Capsella rubella,Eutrema salsugineum,Noccaea caerulescens

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ICE1 sequence

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: ICE1

Background: ICE1 (Inducer of CBF expression 1) is a negative regulator in brassinosteroid signal transduction pathway important for plant growth. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates BHLH150, beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues. Upon brassinosteroid signaling, inhibits stomatal development by phosphorylating and inhibiting the MAPKK kinase YDA and the MAPK kinases MKK4 and MKK5.

Molecular Weight: 43 kDa

Gene ID: 822287

UniProt: Q9LSE2