LexA antibody

Catalog No. ABIN7833406

The Rabbit Polyclonal anti-LexA antibody is suitable to detect LexA in samples from E. coli. It has been validated for WB, ChIP and IL.

Quick Overview for LexA antibody (ABIN7833406)

Target: LexA

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunolocalization (IL)

Product Details

Purpose: Anti-LexA | LexA repressor

Cross-Reactivity (Details): Escherichia coli

Immunogen: Full-size highly-purified recombinant LexA protein

Application Details

Application Notes: 1: 1000 - 1: 3000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Serum. Contains 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details

Target: LexA

Background:

Background: LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.Alternative names: exrA, spr, tsl, umuA

Additional information: This product can be used in:Studies of SOS regulation in E.coliDetection of bait constructs in yeast two-hybrid systemImmunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde)Immunoprecipiation and ChIP

Molecular Weight: 22.3 | 23 kDa

Gene ID: 948544

UniProt: P0A7C2