mCherry antibody

Catalog No. ABIN7833419

The Rabbit Polyclonal anti-mCherry antibody (ABIN7833419) specifically detects mCherry in WB and ICC. The antibody is reactive with Discosoma samples.

Quick Overview for mCherry antibody (ABIN7833419)

Target: mCherry (mCherry Fluorescent Protein)

Reactivity: Discosoma

Host: Rabbit

Clonality: Polyclonal

Conjugate: This mCherry antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-mCherry Antibody

Purpose: Anti-mCherry (rabbit antibody)

Cross-Reactivity (Details): mCherry

Predicted Reactivity: mScarlett

Purification: Immunogen affinity purified

Immunogen: Recombinant full length mCherry expressed and purified from E. coli.

Application Details

Application Notes: 1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified in PBS pH 7.4, with 3 % trehalose.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for mCherry

Target: mCherry (mCherry Fluorescent Protein)

Alternative Name: mCherry

Background: MCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28 kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).