LC3B antibody

Catalog No. ABIN7833424

The Rabbit Polyclonal anti-LC3B antibody is suitable to detect LC3B in samples from Zea mays, Tomato, Poplar (Populus), Nicotiana benthamiana, Haematococcus lacustris, Gossypim hirsutum, Chlorococcum sp. 'dorsiventrale', Chlamydomonas reinhardtii, Arabidopsis thaliana and Aponogeton madagascariensis. It has been validated for WB and IL.

Quick Overview for LC3B antibody (ABIN7833424)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Zea mays, Tomato, Poplar (Populus), Nicotiana benthamiana, Haematococcus lacustris, Gossypim hirsutum, Chlorococcum sp. 'dorsiventrale', Chlamydomonas reinhardtii, Arabidopsis thaliana, Aponogeton madagascariensis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-LC3B Antibody

Purpose: Anti-ATG8 | Autophagy-related protein

Cross-Reactivity (Details): Arabidopsis thaliana, Aponogeton madagascariensis, Chlamydononas reinhardtii, Chlorococcum dorsiventrale, Gossypim hirsutum, Haematococcus lacustris, Nicotiana benthamiana, Populus trichocarpa, Solanum lycopersicum, Zea mays
Not reactive in: Cuscuta chinensis, Symbiodinium sp.

Predicted Reactivity: Ananas comosus,Brassica napus,Hordeum vulgare,Micromonas sp.,Nelumbo nucifera,Oryza sativa,Panicum hallii,Parachlorella kessleri,Phoenix dactylifera,Pyrus x bretschneideri,Physcomitrium patens,Pinus sitchensis,Solanum tuberosum,Triticum aestivum,Volvox carteri

Purification: Serum

Immunogen: Fragment of recombinant ATG8 from Chlamydomonas reinhardtii

Application Details

Application Notes: 1 : 1000 (IL), 1 : 1000-1 : 2000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: ATG8

Background:

Background: ATG8 (Autophagy-related protein 8) is involved in degradation and recycling of intracellular components in a process of autophagy. ATG8 is a molecular autophagy marker in Chlamydomonas reinhardtii (Pérez-Pérez et al. 2010, Plant Physiol. 152: 1874-88).

Additional information: This product can be sold containing ProClin if requested.This antibody is recognizing 1 ng of recombinant CrATG8. Antigen used to elicit this antibody is conserved from 70-80 % in following ATG protein from Arabidopsis thaliana: ATG8a UniProt: Q8LEM4 ATG8B UniProt: Q9XEB5 ATG8c UniProt: Q8S927 ATG8d UniProt: Q9SL04, ATG8e UniProt: Q8S926 ATG8f UniProt: Q8VYK7 and conserved below 70 % in: ATG8g UniProt: Q9LZZ9 ATG8h Uniprot: Q8S92This antibody does not recognize all isoforms into the same degree.

Molecular Weight: 15.2 | 15 kDa

Gene ID: 5724800

UniProt: A8JB85

Pathways: Autophagy