NAD-ME antibody

Catalog No. ABIN7833431

The Rabbit Polyclonal anti-NAD-ME antibody (ABIN7833431) specifically detects NAD-ME in WB, IP and IL. The antibody is reactive with Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria, Nicotiana tabacum, Soybean and Zea mays samples.

Quick Overview for NAD-ME antibody (ABIN7833431)

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Reactivity: Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria, Nicotiana tabacum, Soybean, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAD-ME antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunolocalization (IL)

Product Details anti-NAD-ME Antibody

Purpose: Anti-NAD-ME | Mitochondrial NAD-dependent malic enzyme

Cross-Reactivity (Details): Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Glycine max, Nicotiana tabacum, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Amborella trichopoda,Arabis alpina,Beta vulgaris,Brachypodium distachyon,Brassica oleracea,Citrus sinensis,Coffea canephora,Cucumis sativus,Cynara cardunculus,Erythranthe guttata,Eucalyptus grandis,Eutrema salsugineum,Gossypium raimondii,Hordeum vulgare,Jatropha curcas,Kalanchoe fedtschenkoi,Malus domestica,Medicago truncatula,Morus notabilis,Oryza sativa,Phaseolus vulgaris,Physcomitrium patens,Picea sitcHensis,Populus trichocarpa,Prunus persica,Ricinus communis,Selaginella moellendorffii,Setaria italica,Solanum lycopersicum,Solanum tuberosum,Sorghum bicolor,Spinacia oleracea,Theobroma cacao,Triticum aestivum,Zostera marina,Wollemia nobilis,Vitis vinifera

Immunogen: Native

Application Details

Application Notes: 1 : 25 to 1 : 100 (IL), 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum + 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for NAD-ME

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Alternative Name: NAD-ME

Background:

Background: NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation.

Additional information: Antibody can be used in immunolocalization using immunogold TEM.This antibody recognizes the large subunit (MEL) of the NAD-ME.Immunolocalization method is described in Long et al. (1994).

Molecular Weight: 65 kDa

Pathways: Production of Molecular Mediator of Immune Response