Nitrotyrosine antibody

Catalog No. ABIN7833437

The Mouse Monoclonal anti-Nitrotyrosine antibody (Clone EM-30) (ABIN7833437) specifically detects Nitrotyrosine in WB, IHC (fro) and IHC (p).

Quick Overview for Nitrotyrosine antibody (ABIN7833437)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EM-30

Product Details anti-Nitrotyrosine Antibody

Purpose: Anti-Nitrotyrosine (mouse monoclonal) (clone EM-30)

Cross-Reactivity (Details): Nitrotyrosine

Immunogen: NO2-Tyr-CH2-Thyroglobulin

Isotype: IgG1

Application Details

Application Notes: (IHC) paraffin: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia. (IHC) frozen sections: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia. (WB): positive control: NO2-BSA, NO2-HSA.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunoglobulin purified on Protein A.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube,Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical

Background:

Background: Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.

Additional information: This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues