RFP antibody

Catalog No. ABIN7833506

The Rabbit Polyclonal anti-RFP antibody (ABIN7833506) specifically detects RFP in WB and IHC. The antibody is reactive with Discosoma and Aequorea victoria samples.

Quick Overview for RFP antibody (ABIN7833506)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma, Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RFP Antibody

Purpose: Anti-RFP | Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet)

Cross-Reactivity (Details): RFP, mCherry-YFP
Not reactive in: Sequence identity with GFP is very low and it is therefore expected that this antibody does not cross-reacti with GFP protein.

Predicted Reactivity: mRFP,mCherry,tdTomato,mScarlet

Purification: Antigen affinity purified serum

Immunogen: Recombinant protein produced in E.coli corresponding to full lenght mRFP. The sequence used for immunization is also found in other red flourescent proteins like: mCherry/tdTomato/mScarlet and others.

Application Details

Application Notes: 1: 200 (IHC), 1: 2500 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution, add 50 μL, of sterile or deionized water.

Buffer: Antigen affinity purified serum, in PBS pH 7.4

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP

Background: Red fluorescent protein (RFP) once excited is fluorescing red-orange light. The mass of RFP is approximately 25.9 kDa and its excitation maximum is 558 nm and emission maximum is 583 nm. Improved variants of RFP include so called mFruits variants: mCherry, mOrange, mRaspberry.

Molecular Weight: Depends upon fusion partner