RFP antibody (N-Term)

Catalog No. ABIN7833508

The Mouse Monoclonal anti-RFP antibody is suitable to detect RFP in samples from Discosoma. It has been validated for WB, ELISA, IHC, IP and DB.

Quick Overview for RFP antibody (N-Term) (ABIN7833508)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)

Clone: RF5R

Product Details anti-RFP Antibody

Binding Specificity: N-Term

Purpose: Anti-RFP | Red Fluorescent Protein (clone RF5R)

Cross-Reactivity (Details): Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Protein A purified

Immunogen: KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide

Isotype: IgG1

Application Details

Application Notes: 1 : 1000-3000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Total IgG1. Protein A purified in 1 mg/mL in 10 mM PBS, pH 7.2. Contains 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for up to 2 years.

Expiry Date: 24 months

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP

Background:

Background: RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.

Additional information: Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally