V5 Epitope Tag antibody

Catalog No. ABIN7833580

The Mouse Monoclonal anti-V5 Epitope Tag antibody is suitable to detect V5 Epitope Tag. It has been validated for WB, ELISA, IP and IF.

Quick Overview for V5 Epitope Tag antibody (ABIN7833580)

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This V5 Epitope Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-V5 Epitope Tag Antibody

Purpose: Anti-V5 (mouse antibody (monoclonal)

Cross-Reactivity (Details): V5-tagged fusion proteins

Purification: Affinity purified

Immunogen: KLH-conjugated GKPIPNPLLGLDST synthetic peptide

Isotype: IgG1a

Application Details

Application Notes: 1: 50 - 1: 200 (IF), 1: 200 - 1: 2000 (IP), 1: 500 -1 : 5000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5

Target Type: Tag

Background: V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection. It is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). Addition of a V5-Tag to a protein of interest makes it possible to localize a specific gene product in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.

Molecular Weight: Depends upon MW of fused protein