VPS35 antibody

Catalog No. ABIN7833582

This Rabbit Polyclonal antibody specifically detects VPS35 in WB, IF, IP and ELISA. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for VPS35 antibody (ABIN7833582)

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS35 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA

Product Details anti-VPS35 Antibody

Purpose: Anti-VPS35 | Vacuolar protein sorting-associated protein 35B (marker of PVC)

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: Recombinant

Application Details

Application Notes: assay dependent (ELISA), 1: 400 (IF), 1: 100 (IP), 1: 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Total IgG. Protein A purified in PBS, 50 % glycerol. Filter sterilized.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35

Background: VPS35 (Vacuolar Protein Sorting-associated protein 35) plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Also involved in the efficient sorting of seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane. Arabidopsis thaliana has three VPS35 homologs designated VPS35a, VPS35b and VPS35c, and among them,VPS35b plays most important role in these processes. Localised to pre-vacuolar compartments (PVC).

Molecular Weight: 89 | 98 kDa

Gene ID: 843918

UniProt: F4I0P8